DNA测序技术发展及应用的“地铁”线路图 2012-12-04 01:54:20      来源: 生物帮       责编: 转载

 Nature最新发表了一篇名为“The expanding scope of DNA sequencing”的一篇文章,讲述DNA测序技术的发展及其在各个领域中的应用在不断扩大。




1、ChIP-Seq:Comparison, 'anatomic', cell culture, cross-linking, mechanical shearing, immunoprecipitation, DNA extraction, adaptor ligation, PCR and sequencing

2、TRAP:Comparison, genetic manipulation, 'anatomic', cross-linking, affinity purification, RNA extraction, poly(A) selection, reverse transcription, second-strand synthesis, adaptor ligation, PCR and sequencing

3、RNA-Seq:Comparison, 'anatomic', RNA extraction, poly(A) selection, chemical fragmentation, reverse transcription, second-strand synthesis, adaptor ligation, PCR and sequencing

4、Hi-C:Comparison, cell culture, cross-linking, proximity ligation, mechanical shearing, affinity purification, adaptor ligation, PCR and sequencing

5、Immuno-Seq:Perturbation, 'anatomic', DNA extraction, PCR and sequencing

6、Methyl-Seq:Perturbation, genetic manipulation, cell culture, DNA extraction, mechanical shearing, adaptor ligation, bisulfite conversion, PCR and sequencing

7、Synthetic saturation mutagenesis:Variation, genetic manipulation, barcoding, RNA extraction, reverse transcription, PCR and sequencing

8、Exome-Seq:Comparison, 'anatomic',Toal DNA extraction,Hybrid capture,PCR and sequencing


The expanding scope of DNA sequencing

Jay Shendure,  Erez Lieberman Aiden

In just seven years, next-generation technologies have reduced the cost and increased the speed of DNA sequencing by four orders of magnitude, and experiments requiring many millions of sequencing reads are now routine. In research, sequencing is being applied not only to assemble genomes and to investigate the genetic basis of human disease, but also to explore myriad phenomena in organismic and cellular biology. In the clinic, the utility of sequence data is being intensively evaluated in diverse contexts, including reproductive medicine, oncology and infectious disease. A recurrent theme in the development of new sequencing applications is the creative 'recombination' of existing experimental building blocks. However, there remain many potentially high-impact applications of next-generation DNA sequencing that are not yet fully realized.